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digestion with sphi  (New England Biolabs)


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    Structured Review

    New England Biolabs digestion with sphi
    Digestion With Sphi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/digestion+with+sphi/SphI/pmc12915223-264-22-26
    Average 96 stars, based on 183 article reviews
    digestion with sphi - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Group-I intron family in the nuclear ribosomal RNA small subunit genes ofCenococcum geophilum isolates
    Article Snippet: A family of optional group-I introns was found near the 3@ end of the nuclear small subunit rRNA genes in 61 out of 70 isolates of the deuteromycete mycorrhizal fungus Cenococcum geophilum.. DNA sequence polymorphisms among the introns (termed CgSSU introns) from ten of the isolates were studied.. The sequences, ranging in size from 488 to 514 nucleotides, were from 93.2% to 99.6% similar to each other.

    Polymerase Chain Reaction:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. PCR products were purified with the DNA Clean & Concentrator-5 kit (#D4003, Zymo Research) and cloned into the CD27z vector after double digestion with SphI (#R3182S, New England Biolabs) and BspEI (#R0540S, New England Biolabs), using T4 DNA Ligase (# New England Biolabs, M0202S). .. For cloning into the LF28z/LFBBz vector, the respective PCR products and destination backbones underwent double digestion with MluI (#R3198S, New England Biolabs) and NotI (#R3189S, New England Biolabs).

    Purification:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. PCR products were purified with the DNA Clean & Concentrator-5 kit (#D4003, Zymo Research) and cloned into the CD27z vector after double digestion with SphI (#R3182S, New England Biolabs) and BspEI (#R0540S, New England Biolabs), using T4 DNA Ligase (# New England Biolabs, M0202S). .. For cloning into the LF28z/LFBBz vector, the respective PCR products and destination backbones underwent double digestion with MluI (#R3198S, New England Biolabs) and NotI (#R3189S, New England Biolabs).

    Clone Assay:

    Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
    Article Snippet: .. PCR products were purified with the DNA Clean & Concentrator-5 kit (#D4003, Zymo Research) and cloned into the CD27z vector after double digestion with SphI (#R3182S, New England Biolabs) and BspEI (#R0540S, New England Biolabs), using T4 DNA Ligase (# New England Biolabs, M0202S). .. For cloning into the LF28z/LFBBz vector, the respective PCR products and destination backbones underwent double digestion with MluI (#R3198S, New England Biolabs) and NotI (#R3189S, New England Biolabs).



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